Pyrosequencing Method - Search Videos

Pyrosequencing #sequencing #dna

Pyrosequencing #sequencing #dna

Pyrosequencing enables real-time DNA sequencing based on the detection of pyrophosphate released during DNA elongation. A specific pair of PCR primers, one of them biotin labeled, is used to generate a locus-specific amplicon of approximately 200 base pairs (bp) while a sequencing primer is used to sequence the region of interest and quantify ...

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