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Serial block-face scanning electron microscopy (SBF-SEM) utilizes an ultramicrotome inside the SEM chamber to image the ultrastructure of a resin-embedded biological sample in 3D over a large area. A diamond knife slices sections from the sample block and the exposed sample surface is imaged by the electron beam and backscatter electron detector. The cutting and imaging process is automatically repeated until the desired – or entire - stack is acquired in the z direction of the sample. The indiv
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Serial block-face scanning electron microscopy (SBF-SEM) utilizes an ultramicrotome inside the SEM chamber to image the ultrastructure of a resin-embedded biological sample in 3D over a large area. A diamond knife slices sections from the sample block and the exposed sample surface is imaged by the electron beam and backscatter electron detector. The cutting and imaging process is automatically repeated until the desired – or entire - stack is acquired in the z direction of the sample. The indiv
571 viewsMay 22, 2023
FacebookZEISS Microscopy
Learn how the latest Inlens detector technology of the new ZEISS GeminiSEM Family enables simultaneous Inlens secondary electron (SE) and backscatter electron (EsB) imaging. Gather high resolution, surface- and compositional information, even on sensitive samples. Get more details here: https://zeiss.ly/tgi #ZEISS #GeminiSEM #InlensDetection | ZEISS Microscopy
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Learn how the latest Inlens detector technology of the new ZEISS GeminiSEM Family enables simultaneous Inlens secondary electron (SE) and backscatter electron (EsB) imaging. Gather high resolution, surface- and compositional information, even on sensitive samples. Get more details here: https://zeiss.ly/tgi #ZEISS #GeminiSEM #InlensDetection | ZEISS Microscopy
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FacebookZEISS Microscopy
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